LOCUS       40924_45808        6826 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pUT1 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Kasai Y, Oshima K, Ikeda F, Abe J, Yoshimitsu Y, Harayama S.
  TITLE     Construction of self-cloning system in a unicellular green algae, 
            Pseudochoricystis ellipsoidea
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6826)
  AUTHORS   Kasai Y, Oshima K, Ikeda F, Abe J, Yoshimitsu Y, Harayama S.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2014) Contact:Yuki Kasai Chuo University, 
            Biological Science; 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551, Japan
REFERENCE   3  (bases 1 to 6826)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6826)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-DEC-2014) Contact:Yuki Kasai Chuo University, Biological 
            Science; 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      5..960
                     /note="includes promoter region of Pseudochoricystis
                     ellipsoidea beta-tubulin (TUBULIN1) gene"
                     /regulatory_class="promoter"
     CDS             963..2465
                     /codon_start=1
                     /gene="UMPS"
                     /product="uridine monophosphate synthase"
                     /label=UMPS
                     /protein_id="BAR90701.1"
                     /translation="MATSTPSVATAAELKPKEGPITSQEFEELVLRLHEIEAVKFGNFK
                     LKSGLMSPIYIDLRVIVSYPDVLRRVAEVMWHQVSGAAFDVMCGVPYTALPIATCMSLL
                     HGTPMLMRRKEVKEYGTKKAIEGAFKKGQTCLIVEDLVTSGASVMETVEPLEVEGLKVT
                     DVVVLIDREQGGAARMASNGLRLHSAFTLSFIIKTLQAHGLVSAKVADSVAAFIAANQT
                     FTPSAAAPTPSPAAPAQPKRLPFEERASLCQNAAGRKLLELMARKRTNLAVAADVATVE
                     EMLRIADAAGPHIAVFKTHVDIFDKWDDGIATQLRHLADKHEFLIFEDRKFADIGNTVV
                     SQYGGGIYKIADWSDITNAHLVPGPGIIDGLRKVGQEKGRGLLLLAEMSSKGALATGAY
                     TEKVAEAAAANQDFVMGFICQSPAKWATPVPPGLVHMTPGVQLASGSDALGQQYNTPAS
                     VIGQGGSDVIIVGRGIIKAADPAAAAAQYREAGWAAYEATLA"
     gene            963..2465
                     /gene="UMPS"
                     /label=UMPS
     regulatory      2463..3898
                     /note="includes terminator region of Pseudochoricystis 
                     ellipsoidea actin (ACTIN1) gene"
                     /regulatory_class="terminator"
     promoter        complement(3920..3938)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3959..3975)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3983..3999)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4007..4037)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4052..4073)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4361..4949)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5123..5980)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5981..6085)
                     /label=AmpR promoter
     rep_origin      6112..6567
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6708..6724
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6734..6752
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     6785..6801
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagctttgca tggcattcaa caaggtgaag actgtctgat aagttaatgt atgtgtccgc
       61 ttaaaggttc tgttactcct acctcatcct ggtcaaggtc gttttccgtg gagtccggag
      121 agtctgcaaa ggatcgccta gtcttggctg ggggtgctgt tgcaaacaag atatggccct
      181 gcagcacagc ctgaactgct tgaggctgca tgtgcacaaa tagagagctc tagggtccaa
      241 ccgcatgaag ctgaataaca cctttgatcg cccagcgttg cgtctcctac gggaacacag
      301 gtgagtgtgt cagctcagtc aagacacaga aaccttggac gatttttaag tcggtgcatg
      361 ttcatatagg cacgcaataa cagagtagac tacttataga ctggtcatgt tgcaacacct
      421 gccaagattc agtgatgtct gtgtctgaaa ttttgctgca ggtggagtga tgcccaagga
      481 ttcctctgcc tgctggagaa catcatcgag atctcccgcc agcatatttt ctttcccaga
      541 catactctca ggcgagaaat tttgcatgca taacgctgtg cagcatattg agtcactggc
      601 ttttggacgt ctcttcgagg aatgttgact tctataagtc taggagtaca aaggcaaaag
      661 gcaagtctga aaacatcaaa tacttgcagt acaagaagaa agtttccata ggacaatggc
      721 gacgtcagat gatgttttga cgggccctca gctgcacatc cacaacgccc cgcattttga
      781 aagcctaggg tttagaggtg tatagatatc tagcggggtt ggttgaaaat tttacaagga
      841 acgcccccag tcggatgccc tccttgttgt gcagcagcgc ggcaagcctg ggacacaact
      901 tgccgccttt gtacgagagg cactcgacaa ttggccagga ttgtcgaggg aagtcgttca
      961 agatggcaac ttcaactccc tcagtggcca cagctgcgga gctgaaacca aaggaagggc
     1021 ctatcacgtc acaggagttt gaagagctcg tgctgcgttt gcatgaaatc gaggctgtca
     1081 aattcggcaa cttcaagctc aagagcgggc tcatgtcgcc gatctacatc gacctgcgcg
     1141 tcatcgtgtc ctatccggac gtcctgcgca gggtggcgga ggtgatgtgg catcaggtgt
     1201 cgggcgctgc atttgatgtg atgtgtggcg tgccgtacac ggcgctgccc atagccacat
     1261 gcatgtcgct gctgcacggc acgcccatgc tgatgcggcg caaggaggtc aaggagtacg
     1321 gcaccaagaa ggccattgag ggcgccttca agaagggcca gacctgcctc attgtggagg
     1381 acctggtcac cagcggcgcc tccgtcatgg agactgtgga gcccctcgag gtggaggggc
     1441 tgaaggtgac ggatgtggtg gtgctcatag accgggagca gggcggcgcg gcgcgcatgg
     1501 ccagcaacgg gctgcgactg cactccgcgt tcacgctctc cttcatcatc aagacgctgc
     1561 aggcgcacgg ccttgtcagc gctaaggtgg ccgacagcgt cgctgccttc atagccgcca
     1621 accagacctt caccccctct gcagccgccc ccacaccctc acctgccgct cctgcacagc
     1681 ccaaacggct gccatttgag gagcgcgcaa gcctatgtca gaacgcagcc ggcaggaagc
     1741 tgttggagct gatggcgcgc aagcggacca acctggcggt ggcggcggac gtggcgacgg
     1801 tggaggagat gctacgcatt gcggacgcgg ccgggccaca catcgccgtc ttcaagacgc
     1861 acgtggacat cttcgacaag tgggatgatg gcatcgccac ccagctgcgc catctggctg
     1921 acaagcatga gttcttgata ttcgaggaca ggaagtttgc ggacatcggt aacacagtgg
     1981 tgtcgcagta cggcggaggc atctacaaga ttgccgactg gtcagacatc accaatgcac
     2041 acctggtccc tgggccaggc atcatcgatg gtctccgtaa ggtgggccag gagaaggggc
     2101 gcggcctgct gttgctggct gagatgagct ccaagggagc ccttgccaca ggggcctaca
     2161 ccgagaaggt tgctgaagcg gcagctgcca accaggattt tgtcatgggc ttcatctgcc
     2221 agtcaccagc caagtgggcc acaccggtcc ctccaggtct ggtgcacatg acaccgggag
     2281 tgcagctagc gtctgggagc gacgcattgg ggcagcagta caacacacct gcttctgtca
     2341 tcggccaggg cggcagcgat gtcatcatcg tggggagggg aatcattaag gcggcagacc
     2401 ccgctgcagc ggccgcacag tacagagaag ccggctgggc cgcctatgag gccacgctgg
     2461 catgaggtga gcctgaaaca gctttctgtg tctcctgcga atcttatttg cttgcctttc
     2521 acctcacaac actgggctca ttatatggtg agtgggcctt acatctgctg agcagtggtt
     2581 gttttgtttg caacgtcagc attggcattg tcctcattga gacttggact gacatcggtt
     2641 catgcatttg acaggtggat gtttggattg gggaagcctc cagtgccgat tcgcagcaag
     2701 gcagtctgtt gggcgaagga attgttgggg ccctggtgca gatctgggcg atgggagctt
     2761 gcattacaga caggctatgt ggttatggga gcattcttgg cgtctgattc agagcattgg
     2821 tgatagcagt agcagctggg gacagtgcgc ttttaaggtg tgcgtgttgt gaggtgcatg
     2881 gcctccgccc ttgtgcacat cgtctgttgt tgtcgcaccg tctcatggca cagtaggctt
     2941 cttacaagag cagttgttga tagtgagtgc aggctgtttt tggcattatt gtctcaagac
     3001 aatttcagca ttatgttact gcagccagtg tgggtgatga aatgcggatt gcaaggatga
     3061 tgtattcatt tctgcgtcgt gacatgaatc cagtagtaat gttgtgtttt ggagtggttg
     3121 gattctgtgg tttcagacca taccgtgttg acctcaggga gctccttctg gtgaaggctg
     3181 ttcatgttca tgcatgtaat catggcgatt actctgtctc cagctttgat gtactggggc
     3241 attccagcca tgtggtggtc agagtgttat cttgagcaaa tactgttgac atcctttcat
     3301 caaatggaag ggtgtctgtc tgcccacagc cggctgcaca tgcacaacct tctgtctcca
     3361 acacccaggt actatagtat aagtctcagg tgatactcaa gtacagaata tcccatgtgc
     3421 agtgtgaaca aaggctgact gtcctatgtc agtggaggaa gccccctaca accttcccag
     3481 cgctctgacc cgtcattcct ggtatgtgtc acctgatctt gtgccacaga ccctggctcc
     3541 aggaccggca ggatctaatc tgcgaatcct ctcaatgctg acaatgacta gctacacttc
     3601 aattgaaggg gctctgatgt cagacaagga agccagagag ggacatcgac tggagctgtg
     3661 caggcagagc agggcacatc tcagatgttt ctttgataca aagatgaaaa agggagaacc
     3721 ggtgagaggc acagagcaac tgtgcagcaa ttgaaagtat ttacatggtt tgacctcgca
     3781 tcagcctctt agagcccatt aatgcatgca tcagccaaca actatagata ctaccgtaat
     3841 caggtttaag catgatcatg gccacttcta tgtcaaatgt ccagtgctca actcaacaac
     3901 cggtacccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
     3961 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     4021 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     4081 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     4141 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     4201 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     4261 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     4321 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     4381 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     4441 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     4501 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     4561 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     4621 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     4681 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     4741 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     4801 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     4861 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     4921 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     4981 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     5041 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     5101 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     5161 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     5221 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     5281 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     5341 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     5401 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     5461 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     5521 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     5581 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     5641 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     5701 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     5761 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     5821 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     5881 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     5941 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6001 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     6061 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct
     6121 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     6181 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     6241 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     6301 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     6361 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     6421 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     6481 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     6541 ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca actgttggga
     6601 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     6661 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     6721 cagtgagcgc gcgtaatacg actcactata gggcgaattg gagctccacc gcggtggcgg
     6781 ccgctctaga actagtggat cccccgggct gcaggaattc gatatc
//
